Fig. 4
From: Transcriptomic profiling across human serotonin neuron differentiation via the FEV reporter system
Analysis of transcriptomic profiles during human SN differentiation process. A The schematic of sample collection from four stages during SN differentiation for smart RNA-seq (Day0: hPSC stage; Day21: SNP stage; Day28: immature SN stage; Day42: mature SN stage). B Principal component analysis (PCA) score plot for the samples. C–H Gene expressions of pluripotency (C), neurogenesis (D), synapse (E), axon guidance (F) and serotonergic genes (G) at 4 key stages during SN differentiation. H. Volcano plots of the DEGs. The up-regulated, down-regulated and unchanged metabolites are presented by red, blue, and gray dots, respectively. I, J The functional classification of the DEGs (I: day 28 vs. day 21; J day 42 vs. day 28) based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis