Fig. 4

Altered gene and protein expression of cell cycle and apoptosis-related molecules in KRAS (G13C) iPSC-HPCs. a Quantitative RT-PCR analysis of selected genes related to the regulation of cell cycle and apoptosis in expanded HPCs under standard conditions. The representative results obtained with the isogenic pair C2-1 (WT) and R2-1 (G13C) in two independent experiments are presented as mean ± SEM from biological duplicate samples. b Intracellular flow cytometry analysis for the expression levels of indicated molecules in cultured HPCs. Shown are the representative overlay plots for C2-1 and R2-1 samples. Mean fluorescent values are shown for each histogram