Fig. 1

KRAS (G13C) affects iPSC-derived HPCs in generation efficiencies and myeloid differentiation properties. a A schematic overview of hematopoietic progenitor cell (HPC) and myeloid cell differentiation from isogenic RALD-iPSC pairs. After 14 days of hematopoietic differentiation followed by immunophenotypic analysis, Lin⁻CD34⁺CD43⁺ HPCs were sorted and differentiated into myeloid lineages. b Shown are the results obtained with two clones, either of control (WT) or mutant (G13C) iPSCs. The percentage of CD34⁺CD43⁺ events within the lineage marker-negative population is shown. c Comparison of percent values for the given populations between control (C2-1 and C2-2) and mutant (R2-1 and R2-2) groups. Mean ± SEM values are shown (independent experimental replicates: n = 5). Shown are the P values calculated in the statistical analysis based on a Mann–Whitney test. **p < 0.01. d Immunophenotyping of differentiated cells directed towards a monocytic (M-CSF condition) or a granulocytic (G-CSF condition) lineage. Shown are representative plots obtained using the wild-type (WT) C2-1 and the R2-1 carrying KRAS (G13C) derived from patient 2